主要功能
成像功能:對 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 種參數(shù)進行成像分析。測定調(diào)節(jié)性能量耗散 Y(NPQ),反映植物光保護能力,測定非調(diào)節(jié)性能量耗散Y(NO),反映植物光損傷程度。
程序測量功能:可程序測量熒光誘導曲線、快速光曲線和暗弛豫,也可手動測量;在測量過程中能自動分析所有熒光參數(shù)的變化趨勢
AOI 功能:可在測量前或測量后任意選擇感興趣的區(qū)域(AOI),程序?qū)⒆詣訉x擇的 AOI 的數(shù)據(jù)進行變化趨勢分析,并在報告文件中顯示相關(guān) AOI 的數(shù)據(jù)。所有報告文件中顯示的數(shù)據(jù)都可導出到 EXCEL 文件中。
成像異質(zhì)性分析功能:對任意參數(shù)任意時間的成像,可在圖像上任意選取兩點,軟件自動對兩點間的數(shù)據(jù)進行橫向異質(zhì)性分析,并可導出到 EXCEL 文件中。
成像數(shù)據(jù)范圍分析功能:對任意參數(shù)任意時間的成像,可分析任意兩個熒光數(shù)值之間有多少個像素點,多少面積(cm2)。
突變株篩選功能:可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株。
微藻毒理研究功能:可同時測量 96 個微藻樣品(對照和處理組)的光合活性,軟件自動給出處理組樣品相對于對照組的光合抑制百分比。
吸光系數(shù)測量功能:快速測量葉片的吸光系數(shù)。吸光系數(shù)測量光源: 16 個紅光(650 nm)和 16 個近紅外(780 nm)LED,用于測量植物葉片或藻類樣品 PAR 吸光系數(shù)。
光強異質(zhì)性:測量區(qū)域光強異質(zhì)性≤±2%
測量參數(shù)
Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。
應(yīng)用領(lǐng)域
光合作用研究:可以在完全相同的條件下同時對大量樣品進行成像
植物病理學:病斑部位(包括肉眼不可見時)成像以及病斑擴散的時空動力學
植物脅迫生 理學:肉眼不可見脅迫損傷的早期檢測
遺傳育種:出苗后大規(guī)??焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株
突變株篩選:快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等
微藻毒理學:不同毒物濃度多個重復的樣品一次測完,軟件自動計算抑制比率
分子生物學:宏觀水平上檢測樣品的綠色熒光蛋白(GFP)熒光
其它多種擴展研究
選購指南
一、熒光成像 MAXI 版
系統(tǒng)組成:通用型主機,LED 供電單元, LED 光源,CCD,數(shù)據(jù)線,軟件等。
注意:高等植物或真核藻類測量時選擇藍光光源(推薦),藍藻測量請選擇紅光光源。
熒光成像 MAXI 版本 |
二、熒光成像 MINI 版
系統(tǒng)組成:通用型主機(下圖中未顯示),LED 光源(如下圖),CCD,數(shù)據(jù)線,軟件等。
注意:高等植物或真核藻類測量時選擇藍光光源(推薦),藍藻測量請選擇紅光光源。
熒光成像 MINI 版 |
三、熒光成像 MICROSCOPY 版
系統(tǒng)組成:通用型主機,特制顯微鏡,CCD,數(shù)據(jù)線,軟件等。
熒光成像 MICROSCOPY 版 |
葡萄葉片 Y(NPQ) 成像 | 葡萄葉片 PS/50 成像結(jié)果 | MINI 版本成像結(jié)果 |
| | |
顯微版本成像結(jié)果 | 軟件成像界面 | 慢速誘導動力學曲線 |
產(chǎn)地:德國 WALZ
參考文獻
數(shù)據(jù)來源:光合作用文獻 Endnote 數(shù)據(jù)庫,更新至 2021 年 1 月,文獻數(shù)量超過 10000 篇
原始數(shù)據(jù)來源:Google Scholar
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