簡介
OX1LP溶解氧測量儀使用極譜溶解氧電極，可用于測量水相中的光合作用和/或呼吸作用，適用于藻類、植物、動物和細胞器（葉綠體和線粒體）的懸浮液。還可用于監(jiān)測在水相中產生或消耗氧氣的化學和生化反應。溶解氧包配有儀器、附件、軟件和接口。
可提供從100ul（流通）到50ml不同體積的DO電極。水套反應杯內的氧電極可用于測量生物樣品或酶反應的O2消耗（或產生）。
采用簡單的兩點校準， 可在相同的溫度下進行校準和實驗，或者可以在軟件中對不同溫度下的測量進行簡單的溫度校正。隨機附校準所需的所有附件。
測量對象 v 藻類光合作用與呼吸作用 v 細菌呼吸 v 微生物呼吸 v 小型水生動物的呼吸 v 水生昆蟲和幼蟲呼吸 v 線粒體呼吸 v 葉綠體O2的產生 v 細胞懸浮呼吸 v 植物細胞懸浮光合作用 | 應用領域 v 水生植物光合氧代謝的測定 v 抑制劑對線粒體電子傳遞的影響 v 光依賴性葉綠體電子輸運分析 v 溫度對水生動物攝氧量的影響 v 酶動力學耗氧研究 |
基本組件 溶解氧反應杯電極（1,2.5,4,6,30,50ml） 反應杯電極放大器 磁力攪拌器 攪拌棒和O形圈 KCl填充液 膜材料和濾紙 水套用10英尺Excelon管 LabQuest迷你數(shù)據(jù)接口 Logger Pro軟件 定制安裝軟件 操作手冊 可選組件： 向反應杯中添加代謝物的注射器； 10微升，50微升，100微升 樣品溫度測量用熱敏電阻 校準LED光源 Q-Box堅固外殼，用于儲存和運輸所有部件 |
DO電極，帶磁力攪拌器和LED光源 |
不同體積的溶解氧反應杯電極 OX1LP-1 (1ml 有機玻璃) OX1LP-2.5 (2.5ml 玻璃 或有機玻璃) OX1LP-4 (4ml 有機玻璃) OX1LP-6 (6ml 玻璃) OX1LP-30 (30ml 有機玻璃) OX1LP-50 (50ml有機玻璃或玻璃) |
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軟件界面及數(shù)據(jù)
客戶評價
“I have tested my new Qubit system with isolated oyster mitochondria, and was extremely pleased with the results. I can’t believe I was suffering for the whole year with my old chamber. I have tried three different systems based on Clarke-type electrodes including this one, and this is by far the most convenient for use with small sample volumes and gives a very clean, repeatable trace with the best signal to noise ratio I ever had with micro-chambers.”
From Dr. Inna Sokolova, Assistant Professor, Biology Department, University of North Carolina at Charlotte
“我用分離的牡蠣線粒體測試了Qubit系統(tǒng)，對結果非常滿意。我真不敢相信忍受了整整一年舊培養(yǎng)室。我嘗試了三種不同類型的克拉克電極，包括這一種，這是迄今為止測量小樣本很方便的系統(tǒng)，提供了一種非常干凈、可重復的軌跡，且在微型培養(yǎng)室中信噪比很好。”
——北卡羅來納大學生物系助理教授 Dr. Inna Sokolova
發(fā)表文獻
· Lamarre SG et al. Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). (2019) Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051)
· Sirikhachornkit et al. Increasing the Triacylglycerol Content in Dunaliella tertiolecta through Isolation of Starch-Deficient Mutants. J. Microbiol. Biotechnol. 26(5), 854–866 (2016)
· Croston, Tara L., et al. Functional deficiencies of subsarcolemmal mitochondria in the type 2 diabetic human heart. American Journal of Physiology-Heart and Circulatory Physiology 307.1 H54-H65 (2014)
· Sussarellu, Rossana, et al. Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569 (2013).
· Dickinson, Gary H., et al. Environmental salinity modulates the effects of elevated CO2 levels on juvenile hard-shell clams, Mercenaria mercenaria. The Journal of experimental biology 216.14 2607-2618 (2013).
· Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O2 by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).
· Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E. Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).
· Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).
· Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798(2008).
· Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)
· Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis inChlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)
· Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).
產地：加拿大
